Description
Product Overview:
The PCRClean DX purification system utilizes Aline’s proprietary paramagnetic bead technology for rapid high-throughput purification of DNA fragments.
The consistent recovery efficiency of the PCRClean DX for small and large amplicon sizes is superior to traditional filtration PCR cleanup methods. As such, the PCRClean DX is designed to directly replace Beckman Coulter and Agencourt’s Ampure XP without any protocol changes while providing end-users with superior, more efficient adaptor removal in NGS Library Prep and primer-dimer removal.
Product Highlights
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- Flexible clean-up: high recovery of amplicons with option to choose recovery of >100 bp or >60 bp with modified protocol
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Processing time: 15 minutes/96 samples
- Scalable: suitable for tube, 96 and 384-well plate formats
- Compatible with all next generation sequencing platforms (e.g., Illumina, Ion Torrent)
Applications:
- Sanger sequencing post-PCR cleanup
- Genotyping and mutation detection
- DNA Enriching/Concentrating
- Primer Walking
- All other downstream molecular engineering
Datasheet
Protocols
Protocol is exchangeable with that of Ampure XP. No procedure changes needed if you have been using Ampure XP.
Click Protocol-PCRClean DX for protocol.
MSDS for PCRClean DX
References from Journal Publications
1.Perosser et al. Molecular Ecology Resources, (2016) 16, Page 490; DNA barcodes from century-old type specimens using next-generation sequencing
http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12474/pdf
2.Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012
http://www.pnas.org/content/110/9/E808.full.pdf+html
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