Description
Product Details
Aline DNA Normalizer Kit V2 leverages Aline’s proprietary magnetic bead technology featuring fixed DNA binding surfaces. Users can isolate a pre-defined amount of DNA by simply limiting the bead volume added to the reaction. This kit accomplishes DNA normalization during the purification process, eliminating the need for additional quantification and dilution. By consolidating these steps into a single process, the system reduces time, labor, and reagent costs. All DNA fragments from PCR amplification or enzymatic reactions can be normalized and combined for streamlined Next Gen library construction.
Note: The DNA output following the standard protocol is about 100ng per reaction. However, the output can be adjusted by end-users if desired.
Key Features
- Can be used in both PCR product and fragmented genomic DNA normalization
- Dual action: Normalization and PCR clean up enables significant reduction in processing and faster turn-around times.
- No more Picogreens: time and labor saving.
- Excellent dynamic range, as wide as 7 fold difference in input DNA concentration.
- Consistent normalization result.
- Scalable for both tube, 96 well plate formats.
- Manual and automation friendly.
Process Overview
The protocol mainly consists of binding, washing and elution steps, and can be performed directly in the thermal cycling plate and requires no centrifugation or filtration. The process can be automated with a walk away solution for high throughput applications.
- Bind PCR products or genomic DNA to magnetic beads, then separate beads on magnet plate.
- Wash beads with water to remove excessive DNA, nucleotides, salts, and other contaminants.
- Elute DNA into a Tris buffer.
Down Stream Applications
- Library preparations for all Next Generation Sequencing platforms, e.g. Illumina, PacBio.
- Capillary sequencing
- Any other molecular engineering platforms
Data Sheet
View here.
