Description
Product Details
The PCRClean DX purification system utilizes Aline’s proprietary paramagnetic bead technology for the rapid, high-throughput purification of DNA fragments. Engineered for consistency, the system delivers consistent recovery efficiencies for both small and large amplicons that exceed those of traditional filtration-based PCR cleanup methods.
PCRClean DX is engineered as a direct replacement for Beckman Coulter and Agencourt’s AMPure® XP, requiring zero protocol changes while delivering superior adapter and primer-dimer removal. This seamless “drop-in” compatibility allows labs to maintain existing workflows yet achieve enhanced purification efficiency for NGS library prep.
Key Features
- Flexible clean-up: high recovery of amplicons with option to choose recovery of >100 bp or >60 bp with modified protocol
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Processing time: 15 minutes/96 samples
- Scalable: suitable for tube, 96 and 384-well plate formats
- Compatible with all next generation sequencing platforms (e.g., Illumina, Ion Torrent)
Applications
- Sanger sequencing post-PCR cleanup
- Genotyping and mutation detection
- DNA Enriching/Concentrating
- Primer Walking
- All other downstream molecular engineering
Datasheet
Protocols
Protocol is exchangeable with that of Ampure XP. No procedure changes needed if you have been using Ampure XP.
Click Protocol-PCRClean DX for protocol.
MSDS for PCRClean DX
References from Journal Publications
1.Perosser et al. Molecular Ecology Resources, (2016) 16, Page 490; DNA barcodes from century-old type specimens using next-generation sequencing
http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12474/pdf
2.Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012
http://www.pnas.org/content/110/9/E808.full.pdf+html
