Description
Product Details
The PCRClean PB purification system utilizes proprietary paramagnetic bead technology for rapid, high-throughput DNA fragment purification. Specifically optimized for PacBio® SMRT® Sequencing workflows, this system serves as a direct replacement for AMPure® PB without requiring protocol modifications.
PCRClean PB delivers superior efficiency for adapter removal during PacBio® NGS library preparation and enhanced primer-dimer removal across diverse downstream applications. When compared to traditional filtration-based methods, this system ensures higher recovery efficiency across both small and large amplicon sizes, allowing users to maintain existing workflows while achieving improved purification results.
PCRClean PB is a sister product of PCRClean DX.
Key Features
- Flexible clean-up: high recovery of amplicons with the option to choose recovery of >100 bp or >60 bp with modified protocol
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Recovers amplicons greater than 100 bp with consistency and reliability.
- Processing time: 15 minutes/96 samples
- Scalable: tube, 96 and 384 well plate formatting can be used
Platforms
- Directly replaces Beckman Coulter and Agencourt’s Ampure PB
- Performs next generation sequencing using Pacific Biosciences’ platform
Datasheet
Performance is similar to PCRClean DX (Ampure XP direct replacement) Click here.
Storage
- Store beads at 4°C
Stability
- 12 months if stored as specified
Protocols and SDS
Product References in Journal Publications – PCRClean DX
1. Perosser et al. Molecular Ecology Resources, (2016) 16, Page 490; DNA barcodes from century-old type specimens using next-generation sequencing
http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12474/pdf
2. Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012
http://www.pnas.org/content/110/9/E808.full.pdf+html
