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ALine DNA Normalizer V2

United States patent awarded. It is an ALine DNA Normalizer!

To equalize and purify the DNA fragments from varied sources and concentrations to a similar concentration. Wide dynamic range can handle up to 7 fold input DNA concentration variations. Consistent normalization results.

Ask us if we are the original developer and manufacturer; if we are able to provide technical support and have the know-how.

SKU: N-4001 Categories: ,

Product Description

Detail of the product

DNA Normalizer is based on Aline’s patented magnetic bead technology. Our beads have fixed DNA binding surfaces, therefore by limiting the amount of beads added in a given purification reaction, a pre-defined amount of DNA can be isolated based on the customers’ need. DNA normalization is accomplished during this purification process so that additional DNA quantification and dilution are not necessary. Time, labor and reagent cost are greatly saved with our unique normalization purification system. All DNA fragments (in PCR amplification or enzymatic reactions) can be normalized and combined in one simple process for Next Gen library construction.

Note: Expected DNA output using this kit is 100ng. Please specify if you would like to customize the normalized DNA output is other than 100ng DNA.

Process Overview

1. Bind PCR products or genomic DNA to magnetic beads, then separate beads on magnet plate.

2. Wash beads with water to remove excessive DNA, nucleotides, salts, and other contaminants.

3. Elute DNA.

The protocol mainly consists of binding, washing and elution steps, and can be performed directly in the thermal cycling plate and requires no centrifugation or filtration. The process can be automated with a walk away solution for high throughput applications.

Down Stream Applications

  • Library preparations for all Next Generation Sequencing platforms, e.g. Illumina, Ion Torrent, PacBio, Roche/454, SOLiD.
  • Capillary sequencing
  • Any other molecular engineering platforms

Product Highlights

  • Dual action: Normalization and PCR clean up enables significant reduction in processing and faster turn-around times
  • No more Picogreens: time and labor saving
  • Excellent dynamic range, as wide as 7 fold difference in input DNA concentration
  • Consistant normalization result
  • Scalable: Tube, 96 and 384 well plate formats
  • Manual and automation friendly
  • Output can be either 10ng or 100ng DNA (please specify when place an order) with standard protocol

Datasheet
Click here.

For the most updated DNA Normalizer protocol, please contact us at support@alinebiosciences.com

Specification of the product

N4001-T   Trial Size: 100 rxns

N4001-M   1,000rxns

N4001-L    10,000rxns

 

 

 

 

 

 

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