Description
Product Overview:
The PCRClean PB purification system utilizes Aline’s proprietary paramagnetic bead technology for quick high-throughput purification of DNA fragments. It is designed to directly replace Beckman’s Ampure PB without any protocol changes while providing end-users more efficient adaptor removal in Pacific Biosciences’ NGS Library Prep and primer-dimer removal in all other downstream applications. Compared to traditional filtration PCR cleanup methods, the recovery efficiency of the PCRClean PB for small and large amplicon sizes is superior.
PCRClean PB is a sister product of PCRClean DX.
Key Features:
- Flexible clean-up: high recovery of amplicons with the option to choose recovery of >100 bp or >60 bp with modified protocol
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Recovers amplicons greater than 100 bp with consistency and reliability.
- Processing time: 15 minutes/96 samples
- Scalable: tube, 96 and 384 well plate formatting can be used
Platforms:
- Directly replaces Beckman Coulter and Agencourt’s Ampure PB
- Performs next generation sequencing using Pacific Biosciences’ platform
Datasheet:
Performance is similar to PCRClean DX (Ampure XP direct replacement) Click here.
Sizes:
- 5 mL
- 50 mL
- 250 mL
- 450 mL
Storage:
- Store beads at 4°C
Stability:
- 12 months if stored as specified
Protocols and SDS
Product References in Journal Publications – PCRClean DX
1. Perosser et al. Molecular Ecology Resources, (2016) 16, Page 490; DNA barcodes from century-old type specimens using next-generation sequencing
http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12474/pdf
2. Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012
http://www.pnas.org/content/110/9/E808.full.pdf+html
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