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PCRClean DX – Superior Ampure XP alternative

Looking for a direct replacement for Beckman Ampure XP? PCRClean DX is the ONE with high quality and minimal lot to lot variation.

SKU: C-1003 Categories: ,
SKU Product Size Quantity
C-1003-5 5ml 1
C-1003-50 50ml 1
C-1003-250 250ml 1
C-1003-450 450ml 1

Available Options:

Description

A superior direct replacement to Ampure XP (Beckman Coulter).

PCRClean DX purification system utilizes Aline’s proprietary paramagnetic bead technology for quick high-throughput purification of DNA fragments. Aline PCRCean DX is designed to directly replaces Beckman’s Ampure XP without any protocol changes while providing end users superior efficient adaptor removal in NGS Library Prep and primer-dimer removal in all other down stream applications.

PCRClean DX purification system recovers amplicons greater than 100 bp with consistency and reliability. The recovery efficiency of the PCRClean DX for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods and directly replace Ampure XP (Beckman Coulter/Agencourt)

Applications:

  • All Next Generation Sequencing platforms, Illumina, Ion Torrent, Pacific Bio (Aline’s PCRClean PD equivalent to Ampure PB),Roche’s 454
  • Sanger sequencing post-PCR cleanup
  • Genotyping and mutation detection
  • DNA Enriching/Concentrating
  • Primer Walking
  • All other downstream molecular engineering

Product Highlights

    • Flexible Clean-up: High recovery of amplicons with the option to choose recovery of >100 bp or >60 bp with modified protocol
    • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
    • Processing time: 15 minutes/96 samples
    • Scalable: Tube, 96 and 384 well plate formats

Datasheet

Click here.

PCRClean DX pics on web_1.comparison

PCRClean DX pics on web_2.comparison

References from Journal Publications
1.Perosser et al. Molecular Ecology Resources, (2016) 16, Page 490; DNA barcodes from century-old type specimens using next-generation sequencing
http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12474/pdf

2.Sun et al. Proceedings of the National Academy of Sciences of the United States of America, 2012
http://www.pnas.org/content/110/9/E808.full.pdf+html

Specification of the product

Packaging
Volume/Prep 5mL/500-1,000Preps;50mL/5,000-10,000 Preps; 250mL/25,000-50,000Preps
Storage and Stability
Storage Store beads at 2-8°C | DO NOT FREEZE. Store all other components 15-25°C
Stability Stable for 12 months when all components are stored correctly.
Disclaimers
Use Restriction For Research Use Only. Not for Use in Diagnostic Procedures.

Protocols
Protocol is exchangeable with that of Ampure XP. No procedure changes needed if you have been using Ampure XP.

Click Protocol-Protocol-PCRClean DX v2.10_2 (1) for protocol.

 

Do you have any questions about PCRClean DX?



Additional information

Size

250mL, 450mL, 50mL, 5mL

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